Journal: Acta pharmaceutica (Zagreb, Croatia)

Article Title: Punicalagin attenuated allergic airway inflammation via regulating IL4/IL-4Rα/STAT6 and Notch- GATA3 pathways.

doi: 10.2478/acph-2022-0038

Figure Lengend Snippet: Fig. 7. a) Effect of punicalagin on GATA3, Notch 1 and Notch2 mRNA and b) protein expression of GATA3 in BALB/c mice lung tissue (*p < 0.05). c) Effect of different concentrations of punicalagin on the protein levels of NICD 1, NICD 2, Jagged1 and Jagged2. d) Effect of different concentrations of punicalagin on the protein levels of Notch 1 and Notch 2. * represents p < 0.05 compared to control, while as # represents p < 0.05 compared to OVA alone group.

Article Snippet: Antibodies against GATA3, STAT6, p-STAT6, JAK1, p-JAK1 (Cell signalling Technology, USA), IL-4Rα, Jagged1, Jagged2, Notch1 intracellular domain (NICD1), Notch 2 intracellular domain (NICD2), Notch1, Notch 2 and GAPDH were purchased from Santa Cruz Biotechnology, USA.

Techniques: Expressing, Control

Journal: Circulation

Article Title: Smooth Muscle Notch1 Mediates Neointimal Formation After Vascular Injury

doi: 10.1161/circulationaha.108.790485

Figure Lengend Snippet: Figure 1. Expression of Notch receptors in ECs and SMCs. A, Western blot analysis shows the expression of total Notch1 in ECs isolated from hearts of WT, Notch1flox/, smCre-Tg, N1/-, and smN1/ mice. B, Western blot analysis shows the expression of total Notch1 and Notch3 in SMCs isolated from aortas of WT, Notch1flox/, smCre-Tg, N1/, and smN1/ mice. C, Immunofluores- cence staining of Notch1, Notch2, and Notch3 in uninjured carotid artery (left) and ligated injured carotid artery (14 days after ligation; right) of WT mice. Vessels were costained for smooth muscle actin. Smooth muscle actin is stained red, Notch receptors were stained green, and nuclei were stained blue (DAPI). Yellow indicates presence of both red and green staining. White bar20 m. D, Immunofluorescence costaining of smooth muscle actin with activated Notch1 (NICD). Red was SMA, green was NICD, and blue was DAPI. White bar20 m.

Article Snippet: Immunofluorescence for smooth muscle actin (Sigma; A2547), Notch1 (Santa Cruz Biotechnology; sc-6014), activated Notch1 (NICD; Abcam, ab8925), Notch2 (Rockland; 100-401-406), Notch3 (Santa Cruz Biotechnology; sc-7424), cleaved caspase-3 (Cell Signaling Technology; 9664), and proliferating cell nuclear antigen (PCNA) (Laboratory Vision; RB-9055) was performed by standard procedures.

Techniques: Expressing, Western Blot, Isolation, Staining, Ligation

Journal: Acta pharmaceutica (Zagreb, Croatia)

Article Title: Punicalagin attenuated allergic airway inflammation via regulating IL4/IL-4Rα/STAT6 and Notch- GATA3 pathways.

doi: 10.2478/acph-2022-0038

Figure Lengend Snippet: Fig. 7. a) Effect of punicalagin on GATA3, Notch 1 and Notch2 mRNA and b) protein expression of GATA3 in BALB/c mice lung tissue (*p < 0.05). c) Effect of different concentrations of punicalagin on the protein levels of NICD 1, NICD 2, Jagged1 and Jagged2. d) Effect of different concentrations of punicalagin on the protein levels of Notch 1 and Notch 2. * represents p < 0.05 compared to control, while as # represents p < 0.05 compared to OVA alone group.

Article Snippet: Antibodies against GATA3, STAT6, p-STAT6, JAK1, p-JAK1 (Cell signalling Technology, USA), IL-4Rα, Jagged1, Jagged2, Notch1 intracellular domain (NICD1), Notch 2 intracellular domain (NICD2), Notch1, Notch 2 and GAPDH were purchased from Santa Cruz Biotechnology, USA.

Techniques: Expressing, Control

Journal: Acta pharmaceutica (Zagreb, Croatia)

Article Title: Punicalagin attenuated allergic airway inflammation via regulating IL4/IL-4Rα/STAT6 and Notch- GATA3 pathways.

doi: 10.2478/acph-2022-0038

Figure Lengend Snippet: Fig. 6. Punicalagin regulates STAT6 activation. Punicalagin treatment to OVA-induced mice signifi- cantly decreased IL-4Rα, JAK1expressions and STAT6 activation. * represents p < 0.05 compared to control and # represents p < 0.05 compared to OVA alone group.

Article Snippet: Antibodies against GATA3, STAT6, p-STAT6, JAK1, p-JAK1 (Cell signalling Technology, USA), IL-4Rα, Jagged1, Jagged2, Notch1 intracellular domain (NICD1), Notch 2 intracellular domain (NICD2), Notch1, Notch 2 and GAPDH were purchased from Santa Cruz Biotechnology, USA.

Techniques: Activation Assay, Control

Journal: Acta pharmaceutica (Zagreb, Croatia)

Article Title: Punicalagin attenuated allergic airway inflammation via regulating IL4/IL-4Rα/STAT6 and Notch- GATA3 pathways.

doi: 10.2478/acph-2022-0038

Figure Lengend Snippet: Fig. 6. Punicalagin regulates STAT6 activation. Punicalagin treatment to OVA-induced mice signifi- cantly decreased IL-4Rα, JAK1expressions and STAT6 activation. * represents p < 0.05 compared to control and # represents p < 0.05 compared to OVA alone group.

Article Snippet: Antibodies against GATA3, STAT6, p-STAT6, JAK1, p-JAK1 (Cell signalling Technology, USA), IL-4Rα, Jagged1, Jagged2, Notch1 intracellular domain (NICD1), Notch 2 intracellular domain (NICD2), Notch1, Notch 2 and GAPDH were purchased from Santa Cruz Biotechnology, USA.

Techniques: Activation Assay, Control

Journal: Cancers

Article Title: Notch Signaling Suppresses Melanoma Tumor Development in BRAF/Pten Mice

doi: 10.3390/cancers15020519

Figure Lengend Snippet: Histological and immunohistochemical analyses for tumors from BP (BrafCa/+/Pten-/-), BPN1(BrafCa/+/Pten-/-/Notch1-/-) and BPN2 (BrafCa/+/Pten-/-/Notch2-/-) mice. ( A ) H&E staining for the three different types of tumors. ( B ) Immunohistochemical staining with Ki67 antibody. ( C – E ) Immunohistochemical staining with Notch1 and S100A4 antibodies. ( F – H ). Immunohistochemical staining with Notch2 and S100A4 antibodies. Scale bar equals 100 µm.

Article Snippet: Anti-S100 (melanoma tumor marker) (Roche Ventana #790-2914), anti-Ki67 (proliferation marker) (Abcam #ab16667), anti-NOTCH1 (Millipore Sigma 07-1231), anti-NICD (Cell signaling #4147), anti-HES1 (Cell signaling #11988), anti-MAP2 (ThermoFisherSci #PA5-24589, Waltham, MA, USA) and anti-NOTCH2 (Sigma #HPA048743) antibodies were utilized.

Techniques: Immunohistochemical staining, Staining

Journal: Cancers

Article Title: Notch Signaling Suppresses Melanoma Tumor Development in BRAF/Pten Mice

doi: 10.3390/cancers15020519

Figure Lengend Snippet: Notch signaling expression in human primary melanoma TMA. ( A ) Representative hematoxylin and eosin staining (H&E) of the used TMA slides. ( B – D ) A linear regression analysis was used to assess the relationship between different markers and parameters in human primary melanoma tumors: ( B ) Tumor thickness vs. Ki67. ( C ). Tumor thickness vs. (total NOTCH1, nuclear NICD, nuclear HES1, total NOTCH2, or total MAP2). ( D ). Ki67 vs. (total NOTCH1, nuclear NICD, nuclear HES1, total NOTCH2, or total MAP2).

Article Snippet: Anti-S100 (melanoma tumor marker) (Roche Ventana #790-2914), anti-Ki67 (proliferation marker) (Abcam #ab16667), anti-NOTCH1 (Millipore Sigma 07-1231), anti-NICD (Cell signaling #4147), anti-HES1 (Cell signaling #11988), anti-MAP2 (ThermoFisherSci #PA5-24589, Waltham, MA, USA) and anti-NOTCH2 (Sigma #HPA048743) antibodies were utilized.

Techniques: Expressing, Staining